DIFFERENTIAL DETOXIFICATION AND ANTIOXIDANT RESPONSE IN DIVERSE ORGANS OF CORYDORAS PALEATUS EXPERIMENTALLY EXPOSED TO MICROCYSTIN-RR

ELSEVIER SCIENCE BV

Año: 2006 p. 1 - 1

bstract We assessed changes in activities of both detoxification and antioxidant enzymes as well as lipid peroxidation levels in liver, gill, intestine and brain of Corydoras paleatus exposed to dissolved microcystin-RR (MC-RR). Fish were captured at an unpolluted area, transported to the laboratory, and acclimated previous to experiments. Exposures were carried out using MC-RR at 0.5, 2, 5 and 10 g/L. After exposures for 24 h, fish were sacrificed and dissected separating liver, gills, intestine and brain of each fish. Organs were used for enzyme extractions, evaluating both antioxidant and detoxification systems through the assay of glutathione reductase (GR), guaiacol peroxidase (POD), glutathione peroxidase (GPx), catalase (CAT) as well as glutathione S-transferase (GST). Additionally, thiobarbaturic acid (TBA) method was used to evaluate the peroxidation of lipids (LPO). GSTwas inhibited in all studied organs at most MC-RR concentrations used. Activities of GR, POD and G